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fruA [2019-09-16 09:37:58]
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fruA [2019-09-16 09:37:58]

fructose-specific permease of the phosphotransferase system, EIIABC of the PTS
Locus
BSU_14400
Isoelectric point
5.24
Molecular weight
67.01 kDa
Protein length
635 aa Sequence Blast
Gene length
1908 bp Sequence Blast
Function
fructose uptake and phosphorylation
Product
fructose-specific PTS, EIIABC
Essential
no
E.C.
2.7.1.69
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,509,256 1,511,163

The protein

Catalyzed reaction/ biological activity

  • D-fructose + Nπ-phospho-L-histidyl-[protein] --> D-fructose 1-phosphate + L-histidyl-[protein] (according to UniProt)
  • Protein family

  • PTS permease, fructose/ mannitol family PubMed
  • Paralogous protein(s)

    Structure

  • 2R4Q (EIIB domain)
  • Localization

  • cell membrane (homogeneous) PubMed
  • Expression and Regulation

    Operons

    Description

    Regulatory mechanism

  • FruR: repression, (according to DBTBS), in FruR regulon
  • Regulation

  • induced in the presence of fructose (FruR) (according to DBTBS)
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    Biological materials

    Mutant

  • BKE14400 (fruA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATTTTCACTTGTGTTTCCT, downstream forward: _UP4_TAAGAAAAAAAGCGTCCTTG
  • BKK14400 (fruA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATTTTCACTTGTGTTTCCT, downstream forward: _UP4_TAAGAAAAAAAGCGTCCTTG
  • References

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