fruA [2019-09-16 09:37:58]

fructose-specific permease of the phosphotransferase system, EIIABC of the PTS

Locus: BSU_14400

Isoelectric point: 5.24

Molecular weight: 67.01 kDa

Protein length: 635 aa

Gene length: 1908 bp

Function: fructose uptake and phosphorylation

Product: fructose-specific PTS, EIIABC

Essential: no

E.C.: 2.7.1.69

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Phosphotransferase system → Sugar specific PTS proteins
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of fructose
• Groups of genes → Membrane proteins
• Groups of genes → Phosphoproteins → Phosphorylation on a Cys residue
• Groups of genes → Phosphoproteins → Phosphorylation on a His residue

Gene

Coordinates: 1,509,256 1,511,163

The protein

Catalyzed reaction/ biological activity

• D-fructose + N^π-phospho-L-histidyl-[protein] --> D-fructose 1-phosphate + L-histidyl-[protein] (according to UniProt)

Protein family

• PTS permease, fructose/ mannitol family PubMed

Paralogous protein(s)

• ManP

Structure

• 2R4Q (EIIB domain)

Localization

• cell membrane (homogeneous) PubMed

Expression and Regulation

Operons

• Genes: fruR-fruK-fruA
  Description: PubMed

  Regulatory mechanism

  • FruR: repression, (according to DBTBS), in FruR regulon

  Regulation

  • induced in the presence of fructose (FruR) (according to DBTBS)

Biological materials

Mutant

• BKE14400 (fruA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATTTTCACTTGTGTTTCCT, downstream forward: _UP4_TAAGAAAAAAAGCGTCCTTG
• BKK14400 (fruA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATTTTCACTTGTGTTTCCT, downstream forward: _UP4_TAAGAAAAAAAGCGTCCTTG

References

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